Improved antigen detection on western blots
نویسندگان
چکیده
منابع مشابه
Multiplex detection and quantitation of proteins on western blots using fluorescent probes.
The uses of multiplex detection methodologies are dramatically increasing as a means to increase sample throughput and to demonstrate quantitative differences between multiple targets in gene or protein expression analysis. In this study, we investigate the application of multiplex fluorescent detection for three proteins on the same Western blot using a laser-scanning imaging system, the Bio-R...
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Nonradioactive methods for the detection of immobilized protein on Western blots are standard laboratory practice. This is in contrast to nucleic acid blotting, which still commonly involves the use of radioactive probes. Although the nonradioactive detection of antibody (Ab)1 complexes in Western blotting can employ direct detection of Xuorochrome–Ab conjugates, the majority of systems detect ...
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Citrullination is the conversion of peptidylarginine to peptidylcitrulline, which is catalyzed by peptidylarginine deiminases. This conversion is involved in different physiological processes and is associated with several diseases, including cancer and rheumatoid arthritis. A common method to detect citrullinated proteins relies on anti-modified citrulline antibodies directed to a specific che...
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Human memory T cells associated with cutaneous inflammatory responses are characterized by their expression of cutaneous lymphocyte-associated Ag (CLA), a carbohydrate determinant differentially expressed on P-selectin glycoprotein ligand-1 (PSGL-1). Although expression of the CLA epitope on PSGL-1 (CLA(+) PSGL-1) by memory T cells is associated with acquisition of E-selectin ligand activity, i...
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This article describes nonradioactive probing of a Northern blot. The method employs digoxigenin-labeled probes. Antidigoxigenin antibody/alkaline phosphate conjugate, and a chemiluminescent substrate are subsequently used in the detection system.
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ژورنال
عنوان ژورنال: Technical Tips Online
سال: 2001
ISSN: 1366-2120
DOI: 10.1016/s1366-2120(08)70163-6